Article 1 Scope

This standard specifies the seed production technology for the new variety of sea cucumber "Anyuan 1" (Apostichopus japonicus Selenka).

This standard applies to the new variety of sea cucumber "Anyuan 1" that has been artificially bred.

Article 2 Normative References

The provisions in the following documents become provisions of this standard through reference. Any reference document with a date shall not be applicable to this standard in its subsequent amendments (excluding errata) or revised versions. However, it is encouraged that all parties to agreements based on this standard study the use of the latest versions of these documents. The latest version of any referenced document without a date is applicable to this standard.

GB/T 18407.4 Safety and Quality of Agricultural Products - Environmental Requirements for the Origin of Pollution free Aquatic Products

NY 5052 Pollution free Food Marine Aquaculture Water Quality

NY5071 Guidelines for the Use of Harmless Food and Fishing Drugs

Article 3 Environmental Conditions

（1） Seed production site

The seed production site should choose a sea area with clean water quality, no large amount of fresh water injection, and no industrial, agricultural, and domestic pollution. It is best to build an inner bay with less wind and waves, no floating mud, low turbidity, and high transparency.

（2） Water quality conditions

The water used for seed production shall be treated with sedimentation, multi-stage filtration, and biological purification. The water quality of the source shall comply with the regulations of water quality NY 5052, with a salinity of not less than 25 and a temperature of not less than -2 ℃, pH7.6～8.6， Dissolved oxygen is greater than 5mg/L.

Article 4 Source and characteristics of parental reference

（1） The new variety of sea cucumber "Anyuan 1" is preserved in a specific breeding base.

（2） Parent ginseng is a population with excellent breeding traits, stable genetics, and suitable for expansion and promotion.

（3） It is strictly prohibited to use the offspring of close relatives of sea cucumber for breeding and breeding of sea cucumber seedlings.

（4） The main morphological and genetic characteristics of the sea cucumber "Anyuan 1" meet the germplasm standards for the new variety of sea cucumber "Anyuan 1".

Article 5: Pond aquaculture of parental ginseng

（1） Seedling requirements

The specification is not less than 500/500g, and the quality of the seedlings meets the relevant standard requirements.

（2） Site selection

Ponds should be built in enclosed bays or areas below the middle tidal zone, which are free from pollution in nearby sea areas, far from fresh water sources such as estuaries, and have low wind and waves. The pond pond is preferably built in the middle and low tide areas of the intertidal zone.

（3） Pond requirements

Ponds should be built in enclosed bays or areas below the middle tidal zone, which are free from pollution in nearby sea areas, far from fresh water sources such as estuaries, and have low wind and waves. The pond pond is preferably built in the middle and low tide areas of the intertidal zone.

（4） Water quality requirements

The water quality should comply with the regulations of NY5052, with a salinity of 23~36 and a temperature of -2.0 ℃~33 ℃, pH7.6~8.4， Dissolved oxygen is greater than 3.5mg/L.

（5） Seedling density

The density of seedlings is determined by factors such as the size of the seedlings, the number of ginseng reefs, the frequency of water exchange, and whether to feed them with bait. The advertising density is shown in Table 1.

（6） Daily management

1. After 2 to 3 days of seedling release, let 10cm to 15cm of water enter. When the water level reaches its highest point, change the water according to the color of the water, preferably light yellow or light brown. After entering summer sleep, the highest water level should be maintained, and the daily water change should follow the principles of good water quality, low water temperature, and stable salinity. Increase water change after autumn, with a daily water change rate of 10% to 60%. Maintain the highest water level after freezing in winter.

2. Adhere to morning and evening patrols of the pond, observe and inspect the feeding, growth, and activity of sea cucumbers, with a focus on monitoring water temperature, salinity, dissolved oxygen Technical indicators such as pH should be recorded and effective measures should be taken in a timely manner to identify problems.

3. Timely remove debris from the pool and keep the water clean.

4. Diving every 7-15 days to check the condition of sea cucumbers, including the color of the substrate and the thickness of the silt. Measuring the health, length, and weight of sea cucumbers, and checking their growth. And cut open several sea cucumbers to check their intestinal contents.

Article 6: Selection and cultivation of parental reference

（1） Parental selection

Body length greater than 20cm, weight greater than 200g, strong vitality, and no damage.

（2） Temporary care of ginseng

Refer to the Technical Regulations for Artificial Seedling Cultivation of Sea Cucumber "Anyuan 1".

（3） Qinshen promotes ripening

Article 7 Seedling Breeding

Refer to the Technical Regulations for Seedling Cultivation of the New Variety of Sea Cucumber "Anyuan 1"